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KMID : 0380620180500060688
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Korean Journal of Food Science and Technology
2018 Volume.50 No. 6 p.688 ~ p.696
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Red ginseng-derived saponin fraction inhibits lipid accumulation and reactive oxygen species production by activating nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) pathway
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Kim Chae-Young
Kang Bo-Bin
Hwang Ji-Su
Choi Hyeon-Son
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Abstract
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This study aimed to investigate the effects of red ginseng-derived saponin fraction (SF) on lipid accumulation, reactive oxygen species (ROS) production, and nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) signaling during adipocyte differentiation. SF effectively inhibited lipid accumulation, with the downregulation of adipogenic factors such as peroxisome proliferator-activated receptor gamma (PPARγ) and CCAAT/enhancer-binding protein alpha (C/EBPα). A high dose of SF decreased the protein levels of PPARγ and C/EBPα by over 90% compared to the control. SF-mediated downregulation of adipogenic factors was due to the regulation of early adipogenic factors including C/EBPβ and Krüppel-like Factor 2 (KLF2). In addition, SF (200 µg/mg) decreased intracellular ROS generation by 40% during adipocyte differentiation. However, the SF significantly upregulated Nrf2 and its target proteins, hemoxygenase-1 (HO-1) and NADPH dehydrogenase quinone 1 (NQO1). Furthermore, SF (200 µg/mg) promoted the nuclear translocation of Nrf2. The SF-mediated reduction of lipid accumulation was associated with the regulation of the Nrf2/Keap1 pathway.
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KEYWORD
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red ginseng-derived saponin fraction, lipid accumulation, ROS production, Nrf2/Keap1 pathway
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